Sometimes when a VM is cloned from a template (VMware or Hyper-V) the CID can be duplicated between the two machines. Evidence of this can be seen in the Event Viewer (Event ID 4101). This means the two MSDTC services will not be able to communicate with each other.
A possible error message is:
The Microsoft Distributed Transaction Coordinator (MS DTC) has cancelled the distributed transaction
The MSDTC feature of the Windows operating system requires unique CID values to ensure that MSDTC functionality between computers works correctly. Disk duplicate images of Windows installations must have unique CID values or MSDTC functionality may be impaired. This can occur when using virtual hard disks to deploy an operating system to a virtual machine.
This burned me in the past. Jeff has several scenarios that he walks us through, so if you’re using the Distributed Transaction Coordinator, definitely check this out.